Nitric oxide-releasing nanoparticles accelerate wound healing in NOD-SCID mice

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• 作者：Karin Blecher ; BA^a ; ^b ; ^c ; Luis R. Martinez ; PhD^b ; ^d ; ^e ; Chaim Tuckman-Vernon ; BS^c ; Parimala Nacharaju ; PhD^c ; David Schairer ; BA^a ; ^b ; ^c ; Jason Chouake ; BA^a ; ^b ; ^c ; Joel M. Friedman ; MD ; PhD^c ; Alan Alfieri ; MS^f ; Chandan Guha ; MD ; PhD^f ; Joshua D. Nosanchuk ; MD^a ; ^b ; Adam J. Friedman ; MD ; FAAD^a ; ^c ; ^{adfriedm@montefiore.org}
• 关键词：Wound healing ; Nitric oxide ; Nanotechnology ; Diazeniumdiolate ; Immunodeficiency
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：8
• 期：8
• 页码：1364-1371
• 全文大小：1194 K

文摘

Wound healing is a complex process, coordinated by various biological factors. In immunocompromised states wound healing can be interrupted as a result of decreased numbers of immune cells, impairing the production of effector molecules such as nitric oxide (NO). Therefore, topical NO-releasing platforms, such as diethylenetriamine (DETA NONOate), have been investigated to enhance wound healing. Recently, we demonstrated a nanoparticle platform that releases NO (NO-NPs) in a sustained manner, accelerating wound healing in both uninfected and infected murine wound models. Here, NO-NPs were investigated and compared to DETA NONOate in an immunocompromised wound model using non-obese, diabetic, severe combined immunodeficiency mice. NO-NP treatment accelerated wound closure as compared to controls and DETA NONOate treatment. In addition, histological assessment revealed that wounds treated with NO-NPs had less inflammation, more collagen deposition, and more blood vessel formation as compared to other groups, consistent with our previous data in immunocompetent animals. These data suggest that NO-NPs may serve as a novel wound-healing therapy in the setting of immunocompromised states associated with impaired wound healing.

From the Clinical Editor

Wound healing in an immunocompromised host is often incomplete and is a source of major concern in such conditions. This work demonstrates in a murine model that in these settings NO releasing nanoparticles significantly enhance wound healing.