Amelioration of acute myocardial infarction by saponins from flower buds of Panax notoginseng via pro-angiogenesis and anti-apoptosis

详细信息    查看全文

• 作者：Bin-Rui Yang^a ; ¹ ; Kwok-Kuen Cheung^c ; ¹ ; Xin Zhou^b ; Rui-Fang Xie^b ; Pei-Pei Cheng^b ; Shen Wu^b ; Zhong-Yan Zhou^a ; ^b ; Jing Yi Tang^b ; Pui Man Hoi^a ; You-Hua Wang^b ; ^{doctorwyh@163.com" class="auth_mail" title="E-mail the corresponding author} ; Simon Ming-Yuen Lee^a ; ^{simonlee@umac.mo" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Myocardial infarction ; Panax notoginseng flower ; Saponin ; Angiogenesis ; Apoptosis
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：2 April 2016
• 年：2016
• 卷：181
• 期：Complete
• 页码：50-58
• 全文大小：5042 K

文摘

The root of Panax notoginseng is traditionally used as an anti-hemorrhagic agent to promote blood circulation without causing “congealed” blood. Furthermore, the flower of P. notoginseng is a popular, traditional medicine taken daily for the preventing of hypertension and for reducing blood cholesterol profiles. Besides, the flower of P. notoginseng contains a higher level of saponins, particularly protopanaxadiol-type ginsenosides, as compared to the root. However, detailed pharmacological studies on this flower have rarely been conducted.

Material and methods

In this study, the saponins extracted from the flower of P. notoginseng (FS) were examined on the endothelial cell migration assay, chemically induced vascular insufficiency model in zebrafish larvae and myocardial infraction (MI) model in rats, for determination of their pro-angiogenic and therapeutic effects on MI treatment.

Results

Our results demonstrate that FS significantly promoted VEGF-induced migration of human umbilical vein endothelial cells (HUVECs) and partially restored defective intersegmental vessels (ISV) in a chemically induced vascular insufficiency model of zebrafish larvae. When compared to MI group, two weeks post-treatment of FS (25–50 mg/kg/day) induced approximately 3-fold upregulation of VEGF mRNA expression and a concomitant increase in blood vessel density in the peri-infarct area of the heart. Moreover, TUNEL analysis indicates a reduction in the mean apoptotic nuclei per field in peri-infarct myocardium upon FS treatment.

Conclusions

The pro-angiogenic effects of FS demonstrated in in vitro and in vivo experimental models suggest that the purified saponin preparation from flowers of P. notoginseng may potentially provide preventive and therapeutic agent for cardiovascular diseases.