Effects of Panax notoginseng saponins on proliferation and differentiation of rat embryonic cortical neural stem cells

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• 作者：Yinchu Si^a ; Jun Zhu^b ; Xiang Huang^c ; Peichun Zhu^a ; Chune Xie^d ; ^{chunexie@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：basic fibroblast growth factor ; brain-derived neurotrophic factor ; cell culture ; embryonic rat ; neural stem cells ; Panax notoginseng saponins
• 刊名：Journal of the Chinese Medical Association
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：79
• 期：5
• 页码：256-263
• 全文大小：1048 K

文摘

We aimed to study the effect of Panax notoginseng saponins (PNS) on the proliferation, differentiation, self-renewal, and expressions of basic fibroblast growth factor (bFGF) and brain-derived neurotrophic factor (BDNF) in rat embryonic neural stem cells (NSCs).

Methods

Cortical stem cells were isolated from rat embryos on Embryonic Day 17 (E17) and identified by nestin expression. Subsequently, primary culture, subculturing, and single cell cloning were performed on the cells. After the first cell passage (P1), the cells were resuspended and divided into a control group and a treatment group. Control cells were cultured in serum-free basal culture medium with B27 and dulbecco's modified eagle medium (DMEM)/F12. The same medium supplemented with PNS (100 μg/mL) was used to culture cells in the treatment group. Both groups were incubated at 37°C in a 5% CO₂ incubator. Immunocytochemistry was performed 4 days after incubation.

Results

Primary, P1, and P2 cells in the treatment group formed neurospheres, as did single cell clones of the P1 cells in this group. After being cultured for 4 days, the number of nestin-, proliferating cell nuclear antigen (PCNA)-, Tuj-1-, neurofilament (NF)-, vimentin-, glial fibrillary acidic protein (GFAP)-, bFGF-, and BDNF-positive cells significantly increased in the treatment group in comparison to the control group. All positively stained cells could form clear clusters.

Conclusion

PNS can promote rat embryonic cortical NSC survival, self-renewal, proliferation, and differentiation through neurotrophic factors by autocrine or paracrine signaling.