Growth and motility of human skin fibroblasts on multilayer strong polyelectrolyte films

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• 作者：Magdalena Wytrwal^a ; Paulina Koczurkiewicz^b ; ^c ; Karol Zrubek^b ; Wiktor Niemiec^d ; Marta Michalik^b ; Bart艂omiej Kozik^e ; Edward Szneler^e ; Andrzej Bernasik^a ; ^f ; Zbigniew Madeja^b ; Maria Nowakowska^e ; Mariusz Kepczynski^e ; ^{kepczyns@chemia.uj.edu.pl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Cell motility ; Polyelectrolyte multilayer (PEM) ; Human skin fibroblasts ; Strong polyelectrolyte ; Poly(allylamine hydrochloride) ; Layer-by-Layer (LbL) ; Atomic force microscopy (AFM) ; Contact angle
• 刊名：Journal of Colloid and Interface Science
• 出版年：2016
• 出版时间：1 January 2016
• 年：2016
• 卷：461
• 期：Complete
• 页码：305-316
• 全文大小：4126 K

文摘

Polyelectrolyte multilayers (PEMs) have found application in modifying material surfaces to make them adhesive or non-adhesive for animal cells. However, PEMs made of strong polyelectrolytes are not fully recognized in the literature. This study focuses on the interplay between the properties of PEM assembled from strong polyelectrolytes and cell adhesion and motility. Strong polycations (with quaternary ammonium groups) and a polyanion (with sulfonate groups) were obtained by modification of poly(allylamine hydrochloride) (PAH). Two types of multilayer films were assembled from these PAH derivatives and used to investigate the behavior of human skin fibroblasts (HSFs). The effect of surface charge, hydrophobicity, and film thickness on adhesion of HSFs in a serum-containing medium was studied with immunofluorescence microscopy. The results showed that adhesion of HSFs was strongly depended on the chemical functions of the terminal layer, whereas the wettability was not important. The surface of PEM can be strongly cytophobic (the quaternary ammonium terminal groups) or strongly cytophilic (the sulfonate terminal groups). Finally, the motile activity of HSFs seeded on glass coated with a varying number of polymer layers was investigated. It was demonstrated using an in vitro model that coating the substrate with only two polymer layers can considerably increase the average speed of HSFs movement and stimulate cell migration into the wound.