Biochemical and kinetic evaluation of the enzymatic toxins from two stinging scyphozoans Nemopilema nomurai and Cyanea nozakii

详细信息    查看全文

• 作者：Yang Yue^a ; ^b ; Huahua Yu^a ; ^{yuhuahua@qdio.ac.cn} ; Rongfeng Li^a ; Ronge Xing^a ; Song Liu^a ; Kecheng Li^a ; Xueqin Wang^a ; Xiaolin Chen^a ; Pengcheng Li^a ; ^{pcli@qdio.ac.cn}
• 关键词：Jellyfish venom ; Enzymatic toxins ; Enzyme kinetics ; Metalloproteinase ; Nemopilema nomurai ; Cyanea nozakii
• 刊名：Toxicon
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：125
• 期：Complete
• 页码：1-12
• 全文大小：1830 K
• 卷排序：125

文摘

Jellyfish N. nomurai and C. nozakii nematocyst venom exhibited significant enzymatic properties. Jellyfish venom metalloproteinase activity and PLA2 activity varied depending on the physicochemical conditions. NnNV metalloproteinases kinetically degraded azocasein with the Vmax value of 11.4 ± 1.2 U/mg/min. NnNV PLA2s exhibited slightly higher catalytic activity on NOBA than that of CnNV with a kcat value of 7.5 × 10−3 min−1.