Disruption of the mitotic kinesin Eg5 gene (Knsl1) results in early embryonic lethality

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• 作者：Muriel Chauviè ; re ; Chantal Kress ; Michel Kress
• 关键词：Mitotic kinesin-5 ; Gene targeting ; Knockout ; Mouse preimplantation lethality
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2008
• 出版时间：8 August 2008
• 年：2008
• 卷：372
• 期：4
• 页码：513-519
• 全文大小：1955 K

文摘

Eg5, a member of the widely conserved kinesin-5 family, is a plus-end-directed motor involved in separation of centrosomes, and in bipolar spindle formation and maintenance during mitosis in vertebrates. To investigate the requirement for Eg5 in mammalian development, we have generated Eg5 deficient mice by gene targeting. Heterozygous mice are healthy, fertile, and show no detectable phenotype, whereas Eg5^−/− embryos die during early embryogenesis, prior to the implantation stage. This result shows that Eg5 is essential during early mouse development and cannot be compensated by another molecular motor.