Crystallization and preliminary X-ray crystallographic analysis of strictosidine synthase from Rauvolfia: the first member of a novel enzyme family
详细信息 查看全文
- 作者：Ma ; Xueyan ; Koepke ; Juergen ; Fritzsch ; G??nter ; Diem ; Ralf ; Kutchan ; Toni M. ; Michel ; Hartmut ; et. al.
- 关键词：Strictosidine synthase ; Crystallization ; Alkaloid biosynthesis ; Strictosidine synthase family
- 刊名：Biochimica et Biophysica Acta - Proteins and Proteomics
- 出版年：2004
- 出版时间：October 1, 2004
- 年：2004
- 卷：1702
- 期：1
- 页码：121-124
- 全文大小：133 K
文摘
Strictosidine synthase is a central enzyme involved in the biosynthesis of almost all plant monoterpenoid indole alkaloids. Strictosidine synthase from Rauvolfia serpentina was heterologously expressed in Escherichia coli. Crystals of the purified recombinant enzyme have been obtained by the hanging-drop technique at 303 K with potassium sodium tartrate tetrahydrate as precipitant. The crystals belong to the space group R3 with cell dimensions of a