l-arabonate and d-galactonate production by expressing a versatile sugar dehydrogenase in metabolically engineered Escherichia coli
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- 作者：Huaiwei Liua ; 1 ; Kris Niñ ; o G. Valdehuesaa ; 1 ; Kristine Rose M. Ramosa ; Grace M. Nisolaa ; Won-Keun Leeb ; Wook-Jin Chunga ; wjc0828@gmail.com" class="auth_mail
- 关键词：E. coli ; d-galactonate ; l-arabonate ; l-arabinose dehydrogenase ; Metabolic engineering
- 刊名：Bioresource Technology
- 出版年：May 2014
- 年：2014
- 卷：159
- 期：Complete
- 页码：455-459
- 全文大小：716 K
文摘
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AraDH was characterized and used in l-arabonate and d-galactonate production.
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Sugar metabolism of E. coli hosts were disrupted to allow sugar acid accumulation.
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Sugar acid yields were as high as 99% in strains carrying AraDH.
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Sugar acids were recovered in their salt forms with high purity and recovery.