Identification by Q-PCR of Trypanosoma cruzi lineage and determination of blood meal sources in triatomine gut samples in M¨¦xico

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• 作者：Gabriela Ibá ; &ntilde ; ez-Cervantes^a ; Alejandro Martí ; nez-Ibarra^b ; Benjamí ; n Nogueda-Torres^a ; Eduardo Ló ; pez-Ordu&ntilde ; a^c ; Ana L. Alonso^c ; Cynthia Perea^c ; Teresa Maldonado^c ; José ; Manuel Herná ; ndez^d ; Gloria Leó ; n-Avila^a ; ^{leonavila60@yahoo.com.mx}
• 关键词：Trypanosoma cruzi ; Feeding sources ; Chagas disease ; Q-PCR ; DTU
• 刊名：Parasitology International
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：62
• 期：1
• 页码：36-43
• 全文大小：1299 K

文摘

Triatomine vectors were collected on human dwellings in Michoac¨¢n M¨¦xico. Blood meal sources were identified by real time polymerase chain reaction (Q-PCR) using DNA extracted from triatomine guts. The assay was performed with one only specific primer set to amplify a fragment of the mitochondrial 12S ribosomal gene from vertebrate species. Also Trypanosoma cruzi parasites were detected in triatomine gut samples by microscopy and the positive infection was tested in mice. In addition T. cruzi discrete taxonomic units (DTUs) were identified by Q-PCR with two sets of primers that amplify the mini-circle region (miniexon) and 18S ribosomal mitochondrial gene. The sequences obtained from 18S ribosomal gene amplifications confirmed the presence of T. cruzi I and II lineages, and provide evidence of the presence of lineage TcIII and TcIV.