Two-dimensional gel electrophoresis image registration using block-matching techniques and deformation models

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• 作者：Alvaro Rodriguez^a ; ^{arodriguezta@udc.es" class="auth_mail} ; Carlos Fernandez-Lozano^a ; Julian Dorado^a ; Juan R. Rabu&ntilde ; al^a ; ^b
• 关键词：2D gel images ; Block-matching ; Deformations ; Electrophoresis ; Optical flow ; Proteomics
• 刊名：Analytical Biochemistry
• 出版年：1 June 2014
• 年：2014
• 卷：454
• 期：Complete
• 页码：53-59
• 全文大小：1579 K

文摘

Block-matching techniques have been widely used in the task of estimating displacement in medical images, and they represent the best approach in scenes with deformable structures such as tissues, fluids, and gels. In this article, a new iterative block-matching technique—based on successive deformation, search, fitting, filtering, and interpolation stages—is proposed to measure elastic displacements in two-dimensional polyacrylamide gel electrophoresis (2D–PAGE) images. The proposed technique uses different deformation models in the task of correlating proteins in real 2D electrophoresis gel images, obtaining an accuracy of 96.6% and improving the results obtained with other techniques. This technique represents a general solution, being easy to adapt to different 2D deformable cases and providing an experimental reference for block-matching algorithms.