Adenosine, but not guanosine, protects vaginal epithelial cells from Trichomonas vaginalis cytotoxicity

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• 作者：Camila Braz Menezes^a ; ¹ ; Amanda Piccoli Frasson^a ; ^b ; ¹ ; Lucia Collares Meirelles^a ; Tiana Tasca^a ; ^{tiana.tasca@ufrgs.br}
• 关键词：Trichomonas vaginalis ; Cytopathogenesis ; NTPDase ; ecto-5&prime ; nucleotidase ; Adenosine ; Guanosine
• 刊名：Microbes and Infection
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：19
• 期：2
• 页码：122-131
• 全文大小：948 K
• 卷排序：19

文摘

Trichomonas vaginalis causes the most common non-viral sexually transmitted disease worldwide. The cytoadherence and cytotoxicity upon the vaginal epithelial cells are crucial for the infection. Extracellular nucleotides are released during cell damage and, along with their nucleosides, can activate purinoceptors. The opposing effects of nucleotides versus nucleosides are regulated by ectonucleotidases. Herein we evaluated the hemolysis and cytolysis induced by T. vaginalis, as well as the extracellular nucleotide hydrolysis along with the effects mediated by nucleotides and nucleosides on cytotoxicity. In addition, the gene expression of purinoceptors in host cells was determined. The hemolysis and cytolysis exerted by all T. vaginalis isolates presented positive Pearson correlation. All T. vaginalis isolates were able to hydrolyze nucleotides, showing higher NTPDase than ecto-5′-nucleotidase activity. The most cytotoxic isolate, TV-LACM6, hydrolyzes ATP, GTP with more efficiency than AMP and GMP. The vaginal epithelial cell line (HMVII) expressed the genes for all subtypes of P1, P2X and P2Y receptors. Finally, when nucleotides and nucleosides were tested, the cytotoxic effect elicited by TV-LACM6 was increased with nucleotides. In contrast, the cytotoxicity was reversed by adenosine in presence of EHNA, but not by guanosine, contributing to the understanding of the purinergic signaling role on T. vaginalis cytotoxicity.