Specific interactions between nucleolipid doped liposomes and DNA allow a more efficient polynucleotide condensation

详细信息    查看全文

• 作者：Francesca Cuomo ; Andrea Ceglie ; Francesco Lopez  ; ;   ; ^{lopez@unimol.it}
• 关键词：Lipoplexes ; Nucleolipids ; Molecular interaction ; Nucleic acids ; Self-assembly ; Zeta potential
• 刊名：Journal of Colloid and Interface Science
• 出版年：2012
• 出版时间：1 January 2012
• 年：2012
• 卷：365
• 期：1
• 页码：184-190
• 全文大小：794 K

文摘

The interactions between cationic liposomes doped with the anionic nucleolipid 1,2-dipalmitoyl-sn-glycero-3-cytidine diphosphate (DP-Cyt) and deoxyribonucleic acid (DNA) were investigated. Toward this goal, new liposomal and lipoplex formulations characterized by the presence of the anionic amphiphile DP-Cyt were proposed. The effects of incorporation of the cytosine functionalized lipid DP-Cyt into the cationic bilayers were analyzed by means of electrophoretic mobility, dynamic light scattering (DLS) and fluorescence spectroscopy techniques. These approaches allowed us to follow the DNA condensation process and to identify specific electrokinetic characteristics of liposome and DNA¨Cliposome complexes formation. Specifically, DP-Cyt liposomes and DNA were shown to form electrically stable or unstable complexes depending on the charge ratio between the phosphate group of DNA and the cationic lipid. Remarkably, a prominent role for DP-Cyt in enhancing the DNA binding capacity on liposomes was demonstrated. Zeta potential experiments performed on systems with different liposomes/DNA ratio showed that the value of the charge neutralization point is a function of the content of the incorporated DP-Cyt. As a whole, our data demonstrate that the association of cationic DP-Cyt doped liposomes with DNA is driven by both electrostatic interaction and additional specific interactions at the polar head level based on the cytidine nucleobase.