Radiosynthesis and in vivo evaluation of N-[¹¹C]methylated imidazopyridineacetamides as PET tracers for peripheral benzodiazepine receptors

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• 作者：Katsuhiko Sekimata ; Kentaro Hatano ; Mikako Ogawa ; Junichiro Abe ; Yasuhiro Magata ; Giovanni Biggio ; Mariangela Serra ; Valentino Laquintana ; Nunzio Denora ; Andrea Latrofa ; Giuseppe Trapani ; Gaetano Liso
• 关键词：Positron emission tomography ; Peripheral benzodiazepine receptor ; Imidazopyridineacetamides ; Radiosynthesis ; Neurodegenerative processes ; Alzheimer disease
• 刊名：Nuclear Medicine and Biology
• 出版年：2008
• 出版时间：April 2008
• 年：2008
• 卷：35
• 期：3
• 页码：327-334
• 全文大小：811 K

文摘

Imidazopyridineacetoamide 5–8, a series of novel and potentially selective peripheral benzodiazepine receptor (PBR) ligands with affinities comparable to those of known PBR ligands, was investigated. Radiosyntheses of [¹¹C]5, 6, 7 or 8 was accomplished by N-methylation of the corresponding desmethyl precursors with [¹¹C]methyl iodide in the presence of NaH in dimethylformamide (DMF), resulting in 25 % to 77 % radiochemical yield and specific activitiy of 20 to 150 MBq/nmol. Each of the labeled compounds was injected in ddY mice, and the radioactivity and weight of dissected peripheral organs and brain regions were measured. Organ distribution of [¹¹C]7 was consistent with the known PBR distribution. Moreover, [¹¹C]7 showed the best combination of brain uptake and PBR binding, leading to its high retention in the olfactory bulb and cerebellum, areas where PBR density is high in mouse brain. Coinjection of PK11195 or unlabeled 7 significantly reduced the brain uptake of [¹¹C]7. These results suggest that [¹¹C]7 could be a useful radioligand for positron emission tomography imaging of PBRs.