Rapid screening of toxic salbutamol, ractopamine, and clenbuterol in pork sample by high-performance liquid chromatography—UV method

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• 作者：Kunping Yan^a ; ^b ; ¹ ; ^{kpyan@nwu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Huiqun Zhang^a ; ¹ ; Wenli Hui^a ; Hongli Zhu^a ; ^b ; Xinbo Li^a ; Fangyi Zhong^a ; Xiu'e Tong^c ; Chao Chen^a ; ^d ; ^{cchen898@nwu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：β-agonist ; clenbuterol ; high-performance liquid chromatography&ndash ; UV ; pork ; ractopamine ; salbutamol
• 刊名：Journal of Food and Drug Analysis
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：24
• 期：2
• 页码：277-283
• 全文大小：576 K

文摘

A rapid and simple high-performance liquid chromatography–UV method was developed for the separation and quantification of salbutamol, ractopamine, and clenbuterol in pork. A mixture of acetonitrile–formic acid–ammonium acetate was used as the mobile phase to separate three β-agonists on a C18 column with gradient. The effects of the addition of formic acid and ammonium acetate to mobile phases on the separation of β-agonists were investigated. These additives can greatly improve the resolution and sensitivity. Under the optimized chromatographic condition, this separation does not need extra sample preparation. Complete baseline separation of three β-agonists was achieved in < 20 minutes; the linear range is 0.2–50 μg/L with a correlation coefficient R² value of > 0.99. Excellent method reproducibility was found by intra- and interday precisions with a relative standard deviation of < 3%. The detection limit (S/N = 3) was found to be <0.05 μg/L; this method can be used for routine screening of the β-agonist residues in foods of animal origin before being identified by confirmatory methods.