Enzymatic grafting of functional molecules to the lignin model dibenzodioxocin and lignocellulose material

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• 作者：Tukayi Kudanga ; Endry Nugroho Prasetyo ; Jussi Sipilä ; Gibson S. Nyanhongo ; Georg M. Guebitz
• 关键词：Laccase ; Enzymatic functionalization ; Dibenzodioxocin ; Lignin
• 刊名：Enzyme and Microbial Technology
• 出版年：2010
• 出版时间：5 March 2010
• 年：2010
• 卷：46
• 期：3-4
• 页码：272-280
• 全文大小：1280 K

文摘

Laccase-mediated grafting of functional molecules presents an eco-friendly approach to functionalize lignocellulose materials. In this study functional molecules in the form of reactive phenolic amines, hydrophobicity enhancing fluorophenols and selected wood preservatives, were for the first time successfully coupled onto the lignin model compound dibenzodioxocin (Db) as demonstrated by HPLC-MS analysis. A 1:1-coupling was demonstrated for various combinations including Db and tyramine (m/z 620.5), Db and 3-O-methyldopamine (m/z 650.5), Db and 4-hydroxy-3-methoxybenzylamine (m/z 636.5), Db and 4-fluoro-2-methylphenol (m/z 609.5), and Db and 2-phenylphenol (m/z 653.5). Fungal laccases from Trametes hirsuta and T. villosa were more efficient in mediating the coupling of tyramine to dibenzodioxocin and beech (Fagus sylvatica) wood than a Bacillus sp. laccase with lower redox potential. This work presents for the first time a model for functionalizing of lignocellulose using the lignin model dibenzodioxocin.