Preventive Effect of Hydrogen Water on the Development of Detrusor Overactivity in a Rat Model of Bladder Outlet Obstruction

详细信息    查看全文

• 作者：Nozomu Miyazaki^a ; ^b ; ^{m-nozomu@ee.ce.nihon-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Osamu Yamaguchi^a ; Masanori Nomiya^a ; Ken Aikawa^c ; Junko Kimura^b
• 关键词：urethra ; bladder neck obstruction ; drinking water ; hydrogen ; oxidative stress
• 刊名：The Journal of Urology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：195
• 期：3
• 页码：780-787
• 全文大小：1762 K

文摘

Bladder ischemia and oxidative stress contribute to the pathogenesis of bladder dysfunction caused by bladder outlet obstruction. H₂ reportedly acts as an effective antioxidant. We investigated whether oral ingestion of H₂ water would have a beneficial effect on bladder function in a rat model of bladder outlet obstruction.

Materials and Methods

H₂ water was made by dissolving H₂ gas in ordinary drinking water using a hydrogen water producing apparatus. The bladder outlet obstruction model was surgically induced in male rats. Rats with obstruction were fed H₂ water or ordinary drinking water. On week 4 postoperatively cystometry was performed. Oxidative stress markers and the bladder nerve growth factor level were determined. Bladder tissues were processed for pharmacological studies and histological analysis.

Results

The micturition interval and micturition volume significantly decreased in obstructed rats given ordinary drinking water. These decreases were significantly suppressed by oral ingestion of H₂ water. Increased post-void residual volume in obstructed rats was significantly reduced by H₂ water. Obstruction led to a significant increase in bladder weight, oxidative stress markers and nerve growth factor. H₂ water significantly suppressed these increases without affecting bladder weight. There was no significant difference in histological findings between rats with bladder obstruction given H₂ water and ordinary drinking water. Decreased responses of detrusor muscle strips from obstructed bladders to KCl, carbachol and electrical field stimulation were reversed by H₂ water ingestion.

Conclusions

Results suggest that H₂ water could ameliorate bladder dysfunction secondary to bladder outlet obstruction by attenuating oxidative stress.