Patients with mild enteropathy have apoptotic injury of enterocytes similar to that in advanced enteropathy in celiac disease

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• 作者：Prasenjit Das<sup>bsup> ; Gaurav P.S. Gahlot<sup>bsup> ; Ritu Mehta<sup>bsup> ; Archita Makharia<sup>asup><sup> ; sup><sup>bsup> ; Anil K. Verma<sup>asup> ; Vishnubhatla Sreenivas<sup>csup> ; Subrat K. Panda<sup>bsup> ; Vineet Ahuja<sup>asup> ; Siddhartha Datta Gupta<sup>bsup> ; Govind K. Makharia<sup>asup><sup> ; sup><sup>govindmakharia@gmail.com" class="auth_mail" title="E-mail the corresponding authorsup><sup> ; sup><sup>govindmakharia@aiims.edu" class="auth_mail" title="E-mail the corresponding authorsup>
• 关键词：Advanced enteropathy ; Celiac disease ; Crypt regeneration ; Gluten-free diet ; Mild enteropathy ; Mucosal apoptosis ; Villous abnormality
• 刊名：Digestive and Liver Disease
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：48
• 期：11
• 页码：1290-1295
• 全文大小：1672 K

文摘

Severity of villous atrophy in celiac disease (CeD) is the cumulative effect of enterocyte loss and cell regeneration. Gluten-free diet has been shown to benefit even in patients having a positive anti-tissue transglutaminase (tTG) antibody titre and mild enteropathy.

sSec_2">Aim

spar0010">We explored the balance between mucosal apoptotic enterocyte loss and cell regeneration in mild and advanced enteropathies.

sSec_3">Methods

spar0015">Duodenal biopsies from patients with mild enteropathy (Marsh grade 0 and 1) (n = 26), advanced enteropathy (Marsh grade ≥2) (n = 41) and control biopsies (n = 12) were subjected to immunohistochemical staining for end-apoptotic markers (M30, H2AX); markers of cell death (perforin, annexin V); and cell proliferation (Ki67). Composite H-scores based on the intensity and distribution of markers were compared.

sSec_4">Results

spar0020">End-apoptotic markers and marker of cell death (perforin) were significantly up-regulated in both mild and advanced enteropathies, in comparison to controls; without any difference between mild and advanced enteropathies. Ki67 labelling index was significantly higher in crypts of mild enteropathy, in comparison to controls, suggesting maintained regenerative activity in the former.

sSec_5">Conclusions

spar0025">Even in patients with mild enteropathy, the rate of apoptosis is similar to those with advanced enteropathy. These findings suggest the necessity of reviewing the existing practice of not treating patients with mild enteropathy.