Assessment of glutathione peroxidase-1 polymorphisms, oxidative stress and DNA damage in sensitivity-related illnesses

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• 作者：Agnese Gugliandolo^a ; ¹ ; Chiara Gangemi^a ; ¹ ; Carlo Calabr&ograve ; ^a ; Mercurio Vecchio^a ; Debora Di Mauro^a ; Marcella Renis^b ; Riccardo Ientile^a ; Monica Curr&ograve ; ^a ; Daniela Caccamo^a ; ^{dcaccamo@unime.it" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Idiopathic environmental intolerances ; Impaired detoxification systems ; Erythrocyte glutathione ; Ubiquinone ; Comet assay ; Antioxidant enzyme polymorphisms
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 January 2016
• 年：2016
• 卷：145
• 期：Complete
• 页码：27-33
• 全文大小：558 K

文摘

Oxidative stress increase is a key event for development of sensitivity-related illnesses (SRIs). The aim of this work was to evaluate the influence of a genetic variant in the antioxidant enzyme glutathione peroxidase (GPx1) on oxidative stress development in SRIs.

<h4 id="absSec_2">Main methodsh4>

GPx1 rs1800668 genotype, as well as glutathione, ubiquinone, and DNA damage were assessed in 34 SRI patients and 36 healthy subjects.

<h4 id="absSec_3">Key findingsh4>

Total glutathione, reduced/oxidized glutathione, and ubiquinone were significantly decreased in cases compared with controls, while DNA fragmentation was significantly increased in patients. However, these differences were not associated to GPx1 genetic background.

<h4 id="absSec_4">Significanceh4>

GPx1 rs1800668 polymorphism does not play a major role in SRI-related oxidative stress development.