Fluorescence bioimaging of intracellular signaling and its clinical application

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• 作者：Yusuke Ohba<sup> ; sup><sup>yohba@med.hokudai.ac.jp" class="auth_mail" title="E-mail the corresponding authorsup> ; Yoichiro Fujioka
• 关键词：BAPTA-AM ; acetoxymethyl ester of 1 ; 2-bis(2-aminophenoxy)ethane-N ; N ; N&prime ; N&prime ; -tetraacetic acid ; BiFC ; bimolecular fluorescence complementation ; CML ; chronic myeloid leukemia ; CFP ; cyan-emitting mutant of green fluorescent protein ; FRET ; Fö ; rster resonance energy transfer ; GAP ; GTPase activating protein ; GEF ; guanine nucleotide exchange factor ; GFP ; green fluorescent protein ; IM ; imatinib ; IP3 ; inositol 1 ; 4 ; 5-triphosphate ; MDC ; monodansylcadaverine ; PI3K ; phosphoinositide 3-kinase ; PI(
• 刊名：Journal of Oral Biosciences
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：58
• 期：4
• 页码：113-119
• 全文大小：2501 K

文摘

Fluorescent proteins have continued to shed light on cell biology since the cDNA of wild type green fluorescent protein was first isolated. Nowadays, these remarkable proteins are useful tools, not only in basic research, but also in clinical medicine.

Highlight

By taking advantage of fluorescent protein-based technologies, we identified a signaling network critical for influenza virus internalization and infection. In addition, we developed a highly sensitive biosensor for monitoring kinase activity that utilizes energy transfer between fluorescent proteins. This has led to a high-performance clinical test that enables the prediction of future therapeutic responses and the risk of acquired drug resistance for each individual patient before beginning molecular target therapy.

Conclusion

Technologies that utilize fluorescent proteins, such as the biosensor presented here, should find increasing applications in clinical medicine.