Significance of polymethylmethacrylate (PMMA) modification by zinc oxide nanoparticles for fungal biofilm formation

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• 作者：Mariusz Cierech^a ; ^{mariusz.cierech@wp.pl" class="auth_mail" title="E-mail the corresponding author} ; Adam Kolenda^a ; Anna M. Grudniak^b ; Jacek Wojnarowicz^c ; Bartosz Woźniak^c ; Marlena Gołaś^d ; Ewa Swoboda-Kopeć^d ; Witold Łojkowski^c ; Elżbieta Mierzwińska-Nastalska^a
• 关键词：Denture stomatitis ; Polymethylmethacrylate ; Zinc oxide nanoparticles ; Candida albicans
• 刊名：International Journal of Pharmaceutics
• 出版年：2016
• 出版时间：20 August 2016
• 年：2016
• 卷：510
• 期：1
• 页码：323-335
• 全文大小：4358 K

文摘

The objective of this study was to obtain a material composite with antifungal properties for dentures to be used as an alternative protocol in denture stomatitis treatment and prevention. Denture stomatitis is still a clinical problem in patients particularly vulnerable to this disease. Composites of PMMA and doped ZnO-NPs (weight concentrations, 2.5%, 5%, 7.5%) and PMMA with sprayed solvothermal and hydrothermal ZnO-NPs were tested. The following investigations of newly formed biomaterials were undertaken: influence on Candida albicans solution, biofilm staining, XTT analysis and a quantitative analysis of adhered C. albicans. These studies evidenced the antifungal activity of both nanocomposites PMMA–ZnO-NPs and the efficacy of sputtering of zinc oxide nanoparticles on the PMMA. The study of the biofilm deposition on the surface showed that antifungal properties increase with increasing concentration of ZnO-NPs. The XTT assay in conjunction with testing the turbidity of solutions may indicate the mechanism by which ZnO-NPs exert their effect on the increased induction of antioxidative stress in microorganism cells. The denture base made of the aforesaid materials may play a preventive role in patients susceptible to fungal infections.

Based on the results obtained a modified treatment of stomatitis Type II (Newton’s classification) complicated by fungal infection was proposed.