Cholera toxin B subunit pentamer reassembled from Escherichia coli inclusion bodies for use in vaccination
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- 作者：Yukihiro Tamaki ; Tetsuya Harakuni ; Rui Yamaguchi ; Takeshi Miyata1 ; Takeshi Arakawa ; tarakawa@comb.u-ryukyu.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：COMP ; cartilage oligomeric matrix protein ; CT ; cholera toxin ; CTA ; cholera toxin A subunit ; CTB ; cholera toxin B subunit ; GdnHCl ; guanidine hydrochloride ; GM1 ; monosialoganglioside GM1 ; HMM ; high-molecular-mass ; IBs ; inclusion bodies ; RP ; refolding procedure
- 刊名：Vaccine
- 出版年：2016
- 出版时间：4 March 2016
- 年：2016
- 卷：34
- 期：10
- 页码：1268-1274
- 全文大小：1135 K
文摘
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Cholera toxin B subunit was refolded from Escherichia coli inclusion bodies.
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The refolded protein was predominantly soluble high-molecular-mass aggregates.
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Contaminant removal efficiently regenerated homogeneous pentamer.
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Regenerated pentamer was functionally indistinguishable from choleragenoid.