Microarray analysis of microRNA expression in liver cancer tissues and normal control

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• 作者：Lianjie Lin ; ^{audreylin73@hotmail.com} ; Yan Lin ; Yu Jin ; Changqing Zheng
• 关键词：miRNAs ; microRNAs ; SOAP ; Short Oligonucleotide Alignment Program ; GEO ; Gene Expression Omnibus ; TPM ; transcript per million ; PCA ; principle component analysis ; IR ; irradiation ; ATM ; Ataxia-telangiectasia mutated
• 刊名：Gene
• 出版年：2013
• 出版时间：10 July, 2013
• 年：2013
• 卷：523
• 期：2
• 页码：158-160
• 全文大小：297 K

文摘

Background

Recently, many studies have focused on microRNAs (miRNAs) expression profiling in liver cancer, due to the ability of these small RNAs to potently influence cellular behavior. In this study, to further investigate the relationship between them, the miRNA expression profiling of the cancer liver tissues and normal liver tissues were compared.

Methods

The datasets of miRNAs microarray in liver cancer and normal control were downloaded from Gene Expression Omnibus. Then the SOAP analysis was performed to identify the differentially expressed miRNAs.

Results

A total of 221 differentially expressed miRNAs were found. Five of them (including hsa-miR-15b, hsa-miR-1975, hsa-miR-199a-3p, hsa-miR-199b-3p and hsa-miR-421) were determined by t-test and may be involved in the pathogenesis of liver cancer.

Conclusion

There differentially expressed miRNAs may be potential molecular markers for liver cancer screening.