Design and testing of multiplex RT-PCR primers for the rapid detection of influenza A virus genomic segments: Application to equine influenza virus
详细信息 查看全文
- 作者：EunJung Lee ; Eun-Ju Kim ; Yeun-Kyung Shin ; Jae-Young Song ; songjysong@korea.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Multiplex RT-PCR ; Influenza A virus ; Equine influenza virus ; H3N8 ; Dual priming oligonucleotide (DPO) ; Reverse transcription polymerase chain reaction (RT-PCR) ; Phylogenetic analysis
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：228
- 期：Complete
- 页码：114-122
- 全文大小：1873 K
文摘
- •
Molecular analyses to design primers of influenza A virus are proposed.
- •
Four multiplex RT-PCR assays to detect EIVs were developed.
- •
The mRT-PCR was evaluated with forty-six influenza A viruses from different hosts.
- •
Few cross-reactivity with other animal influenza viruses was observed in the tests.
- •
The mRT-PCR is expected to have a wide range of applications in monitoring and diagnosing EIVs.