Thermostable β-galactosidases for the synthesis of human milk oligosaccharides
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- 作者：Birgitte Zeuner1 ; Christian Nyffenegger1 ; Jø ; rn Dalgaard Mikkelsen ; Anne S. Meyer ; am@kt.dtu.dk" class="auth_mail" title="E-mail the corresponding author
- 刊名：New Biotechnology
- 出版年：2016
- 出版时间：25 May 2016
- 年：2016
- 卷：33
- 期：3
- 页码：355-360
- 全文大小：308 K
文摘
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Two thermostable GH1 β-galactosidases catalyzed formation of LacNAc from GlcNAc and lactose.
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Two thermostableGH1 β-galactosidases catalyzed formation of LNnT from LNT2 and lactose.
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Highest yields of LacNAc and LNnT were obtained with a GH42 β-galactosidase from B. circulans.
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LacNAc formation was markedly improved by a temperature increase from 40°C to 50°C.