Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems
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- 作者：Barbara Petschachera ; b ; Bernd Nidetzkya ; b ; bernd.nidetzky@tugraz.at" class="auth_mail" title="E-mail the corresponding author
- 关键词：CDW ; cell dry weight ; FucT ; fucosyltransferase ; Fkp ; fucokinase/l-fucose-1-phosphate guanylyltransferase ; FL ; fucosyllactose ; GST ; glutathione S-transferase ; HMO ; human milk oligosaccharide ; MBP ; maltose binding protein
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 October 2016
- 年：2016
- 卷：235
- 期：Complete
- 页码：61-83
- 全文大小：3807 K
文摘
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Human milk oligosaccharides are important ingredients for health-related nutrition.
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Their production via multistep enzymatic cascades and using whole cells is reviewed.
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Recombinant production and properties of prokaryotic fucosyltransferases are summarized.
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Enzymatic fucosylation integrated with metabolism for GDP-l-fucose supply is described.
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Current bottlenecks of fucosylated HMO production are discussed.