Antidermatophytic and antileishmanial activities of essential oils from Lippia gracilis Schauer genotypes

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• 作者：Juliana Oliveira de Melo ; Tamires Aparecida Bitencourt ; Ana L¨²cia Fachin ; Elizangela M¨¦rcia Oliveira Cruz ; Hugo C¨¦sar Ramos de Jesus ; P¨¦ricles Barreto Alves ; Maria de F¨¢tima Arrigoni-Blank ; Suzelei de Castro Franca ; Rene Oliveira Beleboni ; Roberta Pereira Mir ; a Fern ; es ; Arie Fitzgerald Blank ; Ricardo Scher
• 关键词：Medicinal plants ; Thymol ; Carvacrol ; Trichophyton rubrum ; Leishmania chagasi
• 刊名：Acta Tropica
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：128
• 期：1
• 页码：110-115
• 全文大小：596 K

文摘

Lippia gracilis, popularly known in Brazil as ¡®alecrim-de-tabuleiro¡¯, is used for many purposes, especially antimicrobial and antiseptic activities. The leaves of three L. gracilis genotypes, including LGRA-106, LGRA-109 and LGRA-110 were collected from the Active Germplasm Bank located in the ¡°Campus Rural da UFS¡± research farm at the S?o Crist¨®v?o country, Sergipe State, Brazil. The essential oils were obtained from leaves of L. gracilis plants by hydrodistillation. Chemical analysis of the essential oils was performed by gas chromatography-mass spectrometry (GC-MS). The susceptibility of Trichophyton rubrum strains, MYA3108 and TruMDR2, to the two L. gracilis genotypes (LGRA-106 and LGRA-109) essential oils was determined by the serial microdilution method. Leishmanicidal activity of essential oil from LGRA-106 and LGRA-110 was assayed by tetrazolium-dye (MTT) colorimetric method. The oxygenated monoterpene thymol was the main component of the essential oil from genotype LGRA-106, while Carvacrol was more abundant in LGRA-109 and LGRA-110. The concentrations of LGRA-106 and LGRA-109 essential oils that completely eliminate the fungi were determined and these concentrations were similar to those observed for fluconazole, a common antifungal drug. Among the genotype tested, LGRA-106 essential oil exhibited the best fungicidal activity at 46.87 ¦Ìg mL^?1. Regarding to leishmanicidal activity, the IC50, for LGRA-106 and LGRA-110, was 86.32 and 77.26 ¦Ìg mL^?1, respectively. The results showed that L. gracilis essential oil, rich in thymol and thymol itself presented best antidermatophytic activity, while the best leishmanicidal activity was obtained with essential oil from genotype rich in Carvacrol and Carvacrol itself.