Gastroprotective effects of the essential oil of Hyptis crenata Pohl ex Benth. on gastric ulcer models

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• 作者：L¨²cio Ricardo Leite Diniz ; Charliene Freire Xavier Vieira ; Edgleyson Chaves dos Santos ; Glauber Cruz Lima ; Kalynca Kayla Viana Arag?o ; Renata Prado Vasconcelos ; Pamella Cristina da Costa Ara¨²jo ; Yuri de Abreu Gomes Vasconcelos ; Aricl¨¦cio Cunha de Oliveira ; Herm¨®genes David de Oliveira ; Viviane Gomes Portella ; Andrelina Noronha Coelho-de-Souza
• 关键词：Hyptis crenata Pohl ex Benth ; Essential oil ; Gastroprotective effect ; Gastric ulcer
• 刊名：Journal of Ethnopharmacology
• 出版年：2013
• 出版时间：7 October, 2013
• 年：2013
• 卷：149
• 期：3
• 页码：694-700
• 全文大小：565 K

文摘

Ethnopharmacological relevance

Hyptis crenata Pohl ex Benth (Lamiaceae), popularly known as ¡°hortel?-brava¡± or ¡°hortel? do campo¡±, is widely distributed in the northeast of Brazil. In Brazil, the leaves of this plant have been used in traditional medicine for the treatment of gastrointestinal disturbances, including gastric ulcers. In an attempt to experimentally validate this claimed antiulcerogenic activity, the gastroprotective effects of the essential oil extracted from the leaves of the Hyptis crenata Pohl ex Benth (EOHc) were evaluated in recognized gastric ulcer models in mice.

Materials and methods

EOHc was obtained by hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC-MS). Swiss male mice (25-30 g) were used for the studies. The gastric ulcers were induced by oral administration of absolute ethanol or indomethacin 45 min after oral pretreatment with EOHc, vehicle and positive control drugs. One hour after the ulcerative challenges, the stomachs were removed and the area of the lesions was measured. The volume, pH and total acidity of the gastric secretions were determined using the pylorus ligature model. The gastrointestinal motility was measured using gastric emptying and intestinal transit. The ethanol-induced gastric mucus depletion and lipid peroxidation were also analyzed.

Results

Our findings are as follows: A significant inhibition of gastric lesions induced by absolute ethanol was observed in the mice pre-treated with EOHc, at a dose of 30 and 100 and 300 mg/kg (5.56¡À1.51, 2.88¡À0.82 and 1.71¡À0.54 mm², respectively) compared to control group (118.03¡À35.4 mm²). Also, EOHc (300 mg/kg) produced a gastroprotective effect against the gastric lesions induced by indomethacin (16.07¡À4.68 mm²) compared to control group (38.64¡À6.1 mm²). EOHc pretreatment produced a reduction in the ethanol-induced lipid peroxidation from 3.9¡À0.22 to 2.4¡À0.1 ¦Ìmol/mg tissue (EOHc-300 mg/kg and control group, respectively). We also observed that EOHc pretreatment decreased the gastric emptying, but did not alter the intestinal transit ratio, ethanol-induced depletion of the gastric wall mucus or secretion parameters (volume, pH and [H⁺]).

Conclusion

Our data indicate that EOHc exerts a gastroprotective effect, indicated by its significant inhibition of gastric lesions in ethanol- and indomethacin-induced ulcer models, which may be associated with its accelerating effect on gastric emptying and reduction in oxidative damages. Our data suggest a potential therapeutic application for EOHc in the treatment of gastric ulcers.