- 作者：Allan Fern ; o Giovanini ; Tatiana Mir ; a Deliberador ; Jos¨¦ Eduardo Tannuri Nemeth ; Viviane Rozeira Crivellaro ; Giovanna Schirmer Portela ; Marco Antonio de Oliveira Filho ; Melissa Rodrigues de Araujo ; Jo?o Cesar Zielak ; Carla Castiglia Gonzaga
- 关键词：Platelet-rich plasma ; Bone regeneration ; TGF-¦Â1 ; Wnt10b ; CD34
- 刊名：Journal of Cranio-Maxillofacial Surgery
- 出版年：2013
- 出版时间：October, 2013
- 年：2013
- 卷：41
- 期：7
- 页码：e180-e186
- 全文大小：2342 K
Background
This study evaluated the osteoconductive effect of an autograft, in the presence or absence of the L-PRP, using histomorphometric analysis of the bone formed, and we compared the results in the presence of TGF-¦Â1, Wnt10b and CD34 detected by immunohistochemistry.Materials and methods
Two bone defects were produced in the calvaria of 20 rabbits. The defects were treated with autograft and autograft combined with L-PRP. The animals were euthanized at 15 and 40 days post-surgery. Data were analyzed by Student-Newman-Keuls (p?¡Ü?0.05) test for histomorphometric and immunohistochemical interpretation.
Results
The results revealed that the presence of bone matrix was significantly less in the defects treated with L-PRP. These results coincided with changes of the immunolocalization of the TGF-¦Â1. In the L-PRP-free groups the TGF-¦Â1 was restricted to bone matrix while the CD34 was scarce and the Wnt10b occurred in peritrabecular cells. In contrast, in defects that received L-PRP the presence of TGF-¦Â1 occurred in cells, which occupied whole area of defect. These TGF-¦Â1+ cells also were co-expressed to Wnt10b and CD34.
Conclusion
These results suggest that L-PRP induces a cross-reaction between TGF-¦Â1 and Wnt10b, which stimulates the self-renewal and maintenance of CD34+ stem cells immunophenotype, impairing the osteoconductivity properties of the autograft.