MMP-1 and MMP-8 expression in giant-cell fibroma and inflammatory fibrous hyperplasia

详细信息    查看全文

• 作者：Henrique Climeck de Oliveira ; DDS ; MSc^a ; ^{climeck@live.com} ; André ; Tschoeke ; DDS^a ; ^{andre.tschoeke@hotmail.com} (Graduate student) ; Gabriele Claudino da Cruz^a ; ^{gabe-cruz@hotmail.com} (Graduate student) ; Lú ; cia Noronha ; MSc ; PhD^a ; ^{lnno@terra.com.br} ; Rafaela Scariot de Moraes ; DDS ; MSc^b ; ^{rafaela_scariot@yahoo.com.br} ; Ricardo Alves Mesquita ; DDS ; MSc ; PhD^c ; ^{ramesquita@ufmg.br} ; Maria Cá ; ssia Ferreira de Aguiar ; DDS ; MSc ; PhD^c ; ^{cassiafaster@gmail.com} ; Patrí ; cia Carlos Caldeira ; DDS ; MSc ; PhD^c ; ^{pat_caldeira@yahoo.com.br} ; Marina de Oliveira Ribas ; DDS ; MSc ; PhD^a ; ^{marina.ribas@pucpr.br} ; Ana Maria Trindade Gré ; gio ; BPharm ; MSc ; PhD^a ; ^{ana.gregio@pucpr.br} ; Luciana Reis Azevedo Alanis ; DDS ; MSc ; PhD^a ; ^{lrazevedo@yahoo.com} ; Sé ; rgio Aparecido Igná ; cio ; MSc ; PhD^a ; ^{s.ignacio@pucpr.br} ; Jean Nunes dos Santos ; DDS ; MSc ; PhD^d ; ^{jeanpatol@gmail.com} ; Antonio Adilson Soares de Lima ; DDS ; MSc ; PhD^e ; ^{aas.lima@ufpr.br} ; Aline Cristina Batista Rodrigues Johann ; DDS ; MSc ; PhD^a ; ^{aline.johann@pucpr.br} (Profa.)
• 关键词：Collagen ; Metalloproteinase-8 ; Metalloproteinase-1
• 刊名：Pathology - Research and Practice
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：212
• 期：12
• 页码：1108-1112
• 全文大小：828 K
• 卷排序：212

文摘

The aim of this study is to compare the immunoexpression of metalloproteinases 1 and 8 in giant-cell fibroma, inflammatory fibrous hyperplasia and normal mucosa. Twenty-two cases of giant-cell fibroma, inflammatory fibrous hyperplasia and oral mucosa (control) each were subjected to immunohistochemistry using anti-metalloproteinase-1 and anti-metalloproteinase-8 antibodies. Eight images of each case were captured and analysed through the a) application of a count grid to count the number of positive neutrophils, macrophages, lymphocytes, plasma cells, fibroblasts and blood vessels to obtain the percentage of staining and b) semi-automated segmentation quantifying the stained area in square micrometres. Statistical tests included ANOVA Two-way, Kruskal Wallis and Games-Howell, with a significance level of 5%. An increased percentage of metalloproteinase-1-immunopositive blood vessels were observed in giant-cell fibroma (26.6 ± 22.4; p = 0.02) and inflammatory fibrous hyperplasia (34.3 ± 31.5; p = 0.01) compared with the control group (19.6 ± 9.2). No significant differences in inflammatory cells, fibroblasts and total area of metalloproteinase-1 and −8 were noted among the three groups. Metalloproteinase-1 apparently acts within the pathogenesis of giant-cell fibroma and inflammatory fibrous hyperplasia.