Short-term hyperprolactinemia decreases allergic inflammatory response of the lungs

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• 作者：J. Ochoa Amaya^a ; ^b ; E.K. Hamasato^a ; C. Nappo Tobaruela^a ; N. Queiroz-Hazarbassanov^a ; A. Anselmo Franci^d ; J. Palermo Neto^a ; F. Greiffo^c ; E. da Silveira^c ; R. de Paula Vieira^c ; A. Ligeiro de Oliveira^c ; C. de Oliveira Massoco Salles Gomes^a ; L. Freitas Felicio^a
• 刊名：Brain, Behavior, and Immunity
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：49
• 期：supp_S
• 页码：e48
• 全文大小：38 K

文摘

The present study evaluated the effects of short-term hyperprolactinemia induced by domperidone before ovalbumin antigenic challenge on the lung’s allergic inflammatory response.To induce hyperprolactinemia, domperidone was injected in rats at a dose of 5.1 mg kg^-1 per day, i.p., for 5 days. Total and differential leukocyte counts from bronchoalveolar lavage (BAL), femoral marrow lavage (FML), and blood were analyzed. The percentages of mucus and collagen production were evaluated microscopically. Enzyme-linked immunosorbent assays were performed to measure serum corticosterone and prolactin levels and interleukin-4 (IL-4), IL-6, IL-10, and TNF- alpha expression in lung explants.The rats that were subjected to short-term hyperprolactinemia exhibited a decrease in leukocyte counts in bronchoalveolar lavage, cellularity decrease in femoral marrow lavage fluid, a lower percentage of mucus, and an increase in lung IL-4, IL-6, IL-10, and TNF-alpha expression. Hyperprolactinemia induced before antigenic challenge decreased allergic lung inflammation. These data suggest that prolactin may play a role in the pathophysiology of asthma. The present study demonstrates a prospective beneficial side effect of domperidone for asthmatic patients. Authors wish to gratefully acknowledge the following grants: 09/51886-3 and 13/01610-7 from Sao Paulo Research Foundation (FAPESP) and 5984-11-4 from the Coordination for the Improvement of Higher Education (CAPES).