Multi-laboratory evaluation of a PCR method for detection of ruminant DNA in commercial processed animal proteins

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• 作者：På ; l A. Olsvik^a ; ^{pal.olsvik@nifes.no} ; Olivier Fumiè ; re^b ; Rob J.C.F. Margry^c ; Gilbert Berben^b ; Natalia Larsen^a ; Martin Alm^d ; Marc H.G. Berntssen^a
• 关键词：Feed safety ; Fish feed ; Bovine spongiform encephalopathy (BSE) ; Ruminant PAP ; PCR assay ; MELISA-TEK
• 刊名：Food Control
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：73
• 期：part_PB
• 页码：140-146
• 全文大小：843 K
• 卷排序：73

文摘

Fish feeds should not contain PAPs from ruminant species. 19 non-ruminant PAPs were analyzed for ruminant content with PCR by three labs. The PCR results was compared with an immunoassay-based method (MELISA-TEK). Ruminant PAP was detected in five of the 19 non-ruminant samples with both methods. Inter-laboratory differences for the PCR method was detected for three samples.