Functional analysis and crystallographic structure of clotrimazole bound OleP, a cytochrome P450 epoxidase from Streptomyces antibioticus involved in oleandomycin biosynthesis

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• 作者：Linda Celeste Montemiglio^a ; ^b ; ¹ ; Giacomo Parisi^a ; ^b ; ¹ ; Antonella Scaglione^a ; ^b ; Giuliano Sciara^a ; ^b ; ² ; Carmelinda Savino^c ; ^{linda.savino@uniroma1.it" class="auth_mail" title="E-mail the corresponding author} ; Beatrice Vallone^a ; ^b ; ^c ; ^{beatrice.vallone@uniroma1.it" class="auth_mail" title="E-mail the corresponding author}
• 关键词：OleP ; 8.8a-deoxyoleandolide epoxigenase ; P450 ; cytochrome P450 ; EpoK ; Epothilone C&ndash ; D monooxygenase ; PimD ; Pimaricin monooxygenase ; MycG ; Mycinamicin monooxygenase ; CLT ; clotrimazole ; KC ; ketoconazole ; DMSO ; dimethyl sulfoxide ; DEO ; 8.8a-deoxyoleandolide ; LO-DEO ; L-olivosyl-8.8a-deoxyoleandolide ; CTZ&ndash ; OleP ; OleP in complex with CTZ ; rmsd ; root mean square deviation ; M-III ; mycinamicin III ; M-IV ; mycinamicin IV ; M-V ; mycinamicin V ; i.s. ; ionic strength ; SEC ; Size Exclusion Chromatograph
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：1860
• 期：3
• 页码：465-475
• 全文大小：1576 K

文摘

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d="p0005">Report of the structure of OleP P450 epoxidase acting on aliphatic carbons

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d="p0010">P450 epoxidase OleP binds clotrimazole following a simple one-step mechanism.

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d="p0015">In complex with clotrimazole OleP adopts an open conformation.

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d="p0020">Clotrimazole contacts residues involved in substrate recognition.

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