Cytotoxic and antioxidative potentials of ethanolic extract of Eugenia uniflora L. (Myrtaceae) leaves on human blood cells

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• 作者：Francisco Assis Bezerra da Cunha^a ; ^b ; Emily Pansera Waczuk^b ; Antonia Eliene Duarte^a ; ^b ; Luiz Marivando Barros^a ; ^b ; Olusola Olalekan Elekofehinti^c ; Edinardo Fagner Ferreira Matias^d ; José ; Galberto Martins da Costa^d ; Adekunle Adeniran Sanmi^e ; Aline Augusti Boligon^b ; Jo&atilde ; o Batista Teixeira da Rocha^b ; Diogo Onofre Souza^g ; Tha&iacute ; s Posser^b ; ^f ; Henrique Douglas Melo Coutinho^d ; Jeferson Luis Franco^b ; ^f ; Jean Paul Kamdem ; Ph.D.^a ; ^b ; ^g ; ^{kamdemjeanpaul2005@yahoo.fr} ; ^{jpkamdem@gmail.com}
• 关键词：Eugenia uniflora ; Pitanga ; DPPH ; TBARS ; Comet assay ; Polyphenols
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：84
• 期：Complete
• 页码：614-621
• 全文大小：1454 K
• 卷排序：84

文摘

Eugenia uniflora is used in the Brazilian folk medicine to treat intestinal disorders and hypertension. However, scanty information exist on its potential toxicity to human, and little is known on its antioxidant activity in biological system. Hence, we investigated for the first time the potential toxic effects of ethanolic extract (EtOH) of E. uniflora (EEEU) in human leukocytes and erythrocytes, as well as its influence on membrane erythrocytes osmotic fragility. In addition, EEEU was chemically characterized and its antioxidant capacity was evaluated. We found that EEEU (1–480 μg/mL) caused neither cytotoxicity nor DNA damage evaluated by Trypan blue and Comet assay, respectively. EEEU (1–480 μg/mL) did not have any effect on membrane erythrocytes fragility. In addition, EEEU inhibited Fe2+-induced lipid peroxidation in rat brain and liver homogenates, and scavenged the DPPH radical. EEEU presented some polyphenolic compounds with high content such as quercetin, quercitrin, isoquercitrin, luteolin and ellagic acid, which may be at least in part responsible for its beneficial effects. Our results suggest that consumption of EEEU at relatively higher concentrations may not result in toxicity. However, further in vitro and in vivo studies should be conducted to ascertain its safety.