Omentopexy enhances graft function in myocardial cell sheet transplantation
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- 作者：Ryo Suzuki ; Fumiyuki Hattori ; Yuji Itabashi ; Masatoyo Yoshioka ; Shinsuke Yuasa ; Haruko Manabe-Kawaguchi ; Mitsushige Murata ; Shinji Makino ; Kiyokazu Kokaji ; Ryohei Yozu ; Keiichi Fukuda
- 关键词：Myocardial cell sheet ; Transplant ; Infarct ; Regenerative medicine ; Omentopexy
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2009
- 出版时间：18 September 2009
- 年：2009
- 卷：387
- 期：2
- 页码：353-359
- 全文大小：1155 K
文摘
Myocardial cell sheets (MCS) are a potentially valuable tool for tissue engineering aimed at heart regeneration. Several methods have recently been established for the fabrication of MCS. However, the lack of a sufficient blood supply has inhibited functional recovery of the MCS. To address this challenge, we combined MCS transplantation with omentopexy (OP), which utilizes omental tissue as a surgical flap. Rats were divided into five groups: sham, myocardial infarction (MI), MCS transplantation, OP, and MCS + OP. Histologic analysis revealed that MCS + OP drastically reversed MI-induced cardiac remodeling. Echocardiography revealed that MCS increased cardiac function, while OP had a synergistic beneficial effect with MCS transplantation. Immunofluorescence imaging showed that OP increased the survival of transplanted cardiomyocytes, and increased the blood supply through enhancement of angiogenesis and migration of small arteries into the MCS. Taken together, we concluded that OP is a promising strategy for the enhancement of graft function in MCS transplantation.