Store-operated calcium entry and calcium influx via voltage-operated calcium channels regulate intracellular calcium oscillations in chondrogenic cells

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• 作者：Já ; nos Fodor^a ; ¹ ; Csaba Matta^b ; ¹ ; Tamá ; s Olá ; h^a ; Tamá ; s Juhá ; sz^b ; Roland Taká ; cs^b ; Adrienn Tó ; th^a ; Beatrix Dienes^a ; Lá ; szló ; Csernoch^a ; Ró ; za Zá ; ká ; ny^b ; ^{roza@anat.med.unideb.hu}
• 关键词：AM ; acetoxy-methylester ; ARC ; arachidonate-regulated Ca2+-sensitive channel ; ATP ; adenosine triphosphate ; CaMKII ; Ca2+&ndash ; calmodulin dependent protein kinase II ; CPA ; cyclopiazonic acid ; CRAC ; Ca2+ release-activated Ca2+ channel ; CREB ; cAMP-response el
• 刊名：Cell Calcium
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：54
• 期：1
• 页码：1-16
• 全文大小：3130 K

文摘

Chondrogenesis is known to be regulated by calcium-dependent signalling pathways in which temporal aspects of calcium homeostasis are of key importance. We aimed to better characterise calcium influx and release functions with respect to rapid calcium oscillations in cells of chondrifying chicken high density cultures. We found that differentiating chondrocytes express the ¦Á₁ subunit of voltage-operated calcium channels (VOCCs) at both mRNA and protein levels, and that these ion channels play important roles in generating Ca²⁺ influx for oscillations as nifedipine interfered with repetitive calcium transients. Furthermore, VOCC blockade abrogated chondrogenesis and almost completely blocked cell proliferation. The contribution of internal Ca²⁺ stores via store-operated Ca²⁺ entry (SOCE) seems to be indispensable to both Ca²⁺ oscillations and chondrogenesis. Moreover, this is the first study to show the functional expression of STIM1/STIM2 and Orai1, molecules that orchestrate SOCE, in chondrogenic cells. Inhibition of SOCE combined with ER calcium store depletion abolished differentiation and severely diminished proliferation, suggesting the important role of internal pools in calcium homeostasis of differentiating chondrocytes. Finally, we present an integrated model for the regulation of calcium oscillations of differentiating chondrocytes that may have important implications for studies of chondrogenesis induced in various stem cell populations.