Influences of diurnal sampling bias on fixed-point monitoring of plankton biodiversity determined using a massively parallel sequencing-based technique

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• 作者：Satoshi Nagai^a ; ^{snagai@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author} ; Kohsuke Hida^b ; Shingo Urushizaki^b ; Goh Onitsuka^c ; Motoshige Yasuike^a ; Yoji Nakamura^a ; Atushi Fujiwara^a ; Seisuke Tajimi^d ; Katsunori Kimoto^e ; Takanori Kobayashi^a ; Takashi Gojobori^f ; Mitsuru Ototake^a
• 关键词：MPS ; massively parallel sequencing ; NMDS ; non-metric multidimensional scaling ; OTUs ; operational taxonomy units
• 刊名：Gene
• 出版年：2016
• 出版时间：1 February 2016
• 年：2016
• 卷：576
• 期：2
• 页码：667-675
• 全文大小：1511 K

文摘

In this study, we investigated the influence of diurnal sampling bias on the community structure of plankton by comparing the biodiversity among seawater samples (n = 9) obtained every 3 h for 24 h by using massively parallel sequencing (MPS)-based plankton monitoring at a fixed point conducted at Himedo seaport in Yatsushiro Sea, Japan. The number of raw operational taxonomy units (OTUs) and OTUs after re-sampling was 507–658 (558 ± 104, mean ± standard deviation) and 448–544 (467 ± 81), respectively, indicating high plankton biodiversity at the sampling location. The relative abundance of the top 20 OTUs in the samples from Himedo seaport was 48.8–67.7% (58.0 ± 5.8%), and the highest-ranked OTU was Pseudo-nitzschia species (Bacillariophyta) with a relative abundance of 17.3–39.2%, followed by Oithona sp. 1 and Oithona sp. 2 (Arthropoda). During seawater sampling, the semidiurnal tidal current having an amplitude of 0.3 m s^− 1 was dominant, and the westward residual current driven by the northeasterly wind was continuously observed during the 24-h monitoring. Therefore, the relative abundance of plankton species apparently fluctuated among the samples, but no significant difference was noted according to G-test (p > 0.05). Significant differences were observed between the samples obtained from a different locality (Kusuura in Yatsushiro Sea) and at different dates, suggesting that the influence of diurnal sampling bias on plankton diversity, determined using the MPS-based survey, was not significant and acceptable.