Histone H2B gene cloning, with implication for its function during nuclear shaping in the Chinese mitten crab, Eriocheir sinensis
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- 作者：Jiangli Wu ; Shumei Mu ; Mingshen Guo ; Tingrong Chen ; Zhaohui Zhang ; Zhenqiu Li ; Yanqin Li ; Xianjiang Kang ; xjkang@hbu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：HRP ; horseradish peroxidase ; PCR ; polymerase chain reaction ; ORF ; open reading frame ; LB ; Luria&ndash ; Bertani ; OD600 ; optical density at 600  ; nm ; IPTG ; isopropyl β-D-thiogalactopyranoside ; SDS-PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis ; PVDF ; polyvinylidene fluoride ; TBST ; Tris-buffered saline with Tween-20 ; ECL ; enhanced chemiluminescence ; H&E ; hematoxylin and eosin ; OCT ; optimal cutting temperature formulation ; TBS ; Tris-buffered saline ; DAPI ; 4&prime ; 6-diamidino-2-ph
- 刊名：Gene
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：575
- 期：2
- 页码：276-284
- 全文大小：1726 K
文摘
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The first cloning of the histone H2B encoding sequence from Eriocheir sinensis.
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Histone H2B was in the mature sperm nucleus of Eriocheir sinensis.
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The persistence of histone H2B might have association with the uncondensed chromatin.