Spectral properties of stilbazolium merocyanines – potential sensitizers in photodynamic therapy and diagnosis: Part II. Merocyanines in resting and stimulated lymphocytes
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- 作者：Staskowiak ; Ewa ; Dudkowiak ; Alina ; Wiktorowicz ; Krzysztof ; Cofta ; Justyna ; Frackowiak ; Danuta
- 关键词：AV ; annexin-V ; BCP ; bromocresol purple ; BPB ; bromophenol blue ; FITC ; fluorescein ; ISC ; intersystem crossing ; LIOAS ; laser-induced optoacoustic spectroscopy ; Mero ; merocyanine ; PBS ; phosphate buffered saline ; PDD ; photodynamic diagnosis ; PDT ; photodynamic
- 刊名：Journal of Photochemistry & Photobiology, A: Chemistry
- 出版年：2005
- 出版时间：January 15, 2005
- 年：2005
- 卷：169
- 期：2
- 页码：159-168
- 全文大小：311 K
文摘
Three stilbazolium merocyanines, selected on the basis of their investigations in model systems in part I of this work [J. Photochem. Photobiol., A: Chem. 163 (2004) 127], were introduced into resting and phytohemagglutinin-stimulated lymphocytes. All these merocyanines in solution exhibit high yield of generation of very photochemically active triplet states, but are characterized by different yields of fluorescence emission. The PHA-stimulated lymphocytes were the model of tumor cells. The fluorescence emission and fluorescence excitation spectra are more sensitive to small changes in the cell material than absorption spectra. The course of the photodynamic reactions in stained and unstained cells were established.