Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in聽vivo equine skin wound model

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• 作者：Sarah Y. Broeckx¹ ; ² ; Bizunesh M. Borena³ ; ⁴ ; Lore Van Hecke⁵ ; Koen Chiers⁶ ; Sofie Maes⁷ ; Deborah J. Guest⁸ ; Evelyne Meyer⁹ ; Luc Duchateau³ ; Ann Martens⁵ ; Jan H. Spaas¹ ; ² ; ^{jan.spaas@anacura.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：allogeneic ; autologous ; horse ; skin ; stem cells
• 刊名：Cytotherapy
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：17
• 期：10
• 页码：1434-1446
• 全文大小：3112 K

文摘

Several studies report beneficial effects of autologous and allogeneic stem cells on wound healing. However, no comparison between autologous versus allogeneic epithelial-like stem cells (EpSCs) has been made so far. For this reason, we first hypothesize that both EpSC types enhance wound healing in comparison to vehicle treatment and untreated controls. Second, on the basis of other studies, we hypothesized that there would be no difference between autologous and allogeneic EpSCs.

Methods

Twelve full-thickness skin wounds were created in six horses. Each horse was subjected to (i) autologous EpSCs, (ii) allogeneic EpSCs, (iii) vehicle treatment or (iv) untreated control. Wound evaluation was performed at day 3, 7 and 14 through wound exudates and at week 1, 2 and 5 through biopsies.

Results

Wound circumference and surface were significantly smaller in autologous EpSC-treated wounds. A significantly lower amount of total granulation tissue (overall) and higher vascularization (week 1) was observed after both EpSC treatments. Significantly more major histocompatibility complex II–positive and CD20-positive cells were noticed in EpSC-treated wounds at week 2. In autologous and allogeneic groups, the number of EpSCs in center biopsies was low after 1 week (11.7% and 6.1%), decreased to 7.6% and 1.7%, respectively (week 2), and became undetectable at week 5.

Conclusions

These results confirm the first hypothesis and partially support the second hypothesis. Besides macroscopic improvements, both autologous and allogeneic EpSCs had similar effects on granulation tissue formation, vascularization and early cellular immune response.