A method for objectively quantifying propidium iodide exclusion in organotypic hippocampal slice cultures
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- 作者：Denise F. Happa ; R. Andrew Taskera ; b ; tasker@upei.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：CA ; cornu ammonis ; DG ; dentate gyrus ; NMDA ; N-methyl-d-aspartate ; OHSC ; organotypic hippocampal slice cultures ; PI ; propidium iodide ; PND ; postnatal day
- 刊名：Journal of Neuroscience Methods
- 出版年：2016
- 出版时间：30 August 2016
- 年：2016
- 卷：269
- 期：Complete
- 页码：1-5
- 全文大小：529 K
文摘
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Organotypic hippocampal cultures are a great tool to investigate excitotoxicity.
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A simple method to quantify cell death using propidium iodide is proposed.
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This facilitates comparison of responses to drug administration on cell viability.
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Increasing doses of NMDA resulted in a dose-dependent increase in PI uptake.