A rapid method for the separation of vitamin D and its metabolites by ultra-high performance supercritical fluid chromatography-mass spectrometry
详细信息 查看全文
- 作者：Firas Jumaaha ; Sara Larssonb ; Sofia Essé ; na ; Larissa P. Cunicoa ; Cecilia Holmb ; Charlotta Turnera ; Margareta Sandahla ; Margareta.Sandahl@chem.lu.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ultra-high performance supercritical fluid chromatography ; Vitamin D2 and D3 metabolites ; Orthogonal column screening ; Method development ; Plasma ; Mass spectrometry
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：1440
- 期：Complete
- 页码：191-200
- 全文大小：1783 K
文摘
- •
Column screening on six orthogonal stationary phases for vitamin D analysis.
- •
Nine vitamin D metabolites (D2 and D3 forms) are baseline separated in 8 min.
- •
The effect of co-solvent, temperature, pressure and flow rate is studied.
- •
ESI+ is 6-fold more sensitive than APCI+ in SFC–MS.