Biochemical properties of acid-soluble collagens extracted from the skins of underutilised fishes

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• 作者：Inwoo Bae ; Kiyoshi Osatomi ; Asami Yoshida ; Kazufumi Osako ; Atsuko Yamaguchi ; Kenji Hara
• 关键词：Collagen ; Underutilised fish ; Ray ; Skin ; Denaturation temperature
• 刊名：Food Chemistry
• 出版年：2008
• 出版时间：1 May 2008
• 年：2008
• 卷：108
• 期：1
• 页码：49-54
• 全文大小：279 K

文摘

Acid-soluble collagens (ASCs) were extracted from the skins of several underutilised fishes, namely dusky spinefoot (Siganus fuscescens), sea chub (Kyphosus bigibbus), eagle ray (Myliobatis tobijei), red stingray (Dasyatis akajei) and yantai stingray (Dasyatis laevigata). The yields of the ASCs from skins of dusky spinefoot and sea chub were about 3.4–3.9 % , and from ray species were about 5.3–5.7 % , on a dry weight basis. According to the electrophoretic pattern, ASCs consisted of two different α-chains (α1 and α2) and were classified as type I collagen. However, the molecular weights of α2-chain for ray species were lower than those of bony fishes. ASC from ray species contained a higher content of imino acids than those from dusky spinefoot and sea chub. The denaturation temperatures (T_d) of ray species were about 33 °C, which was about 5 °C higher than those of dusky spinefoot and sea chub. The high T_d of ray species suggested the possibility of its utilisation as a substitute for mammalian collagen.