Quantitative histological assessment of hepatic ischemia-reperfusion injuries following ischemic pre- and post-conditioning in the rat liver

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• 作者：Anders R. Knudsen ; MD ; PhD^a ; ^{auknudsen@gmail.com} ; Anne-Sofie Kannerup ; MD ; PhD^a ; Henning Gr&oslash ; nbæ ; k ; MD ; PhD^b ; Stephen H. Dutoit ; DMSc^c ; Jens R. Nyengaard ; DMSc^d ; ^e ; Peter Funch-Jensen ; DMSc^e ; Frank V. Mortensen ; DMSc^a
• 关键词：Reperfusion injury ; Liver failure ; Acute ; Warm ischemia ; Stereology ; Quantitative microscopy
• 刊名：Journal of Surgical Research
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：180
• 期：1
• 页码：e11-e20
• 全文大小：1211 K

文摘

Background

Ischemic preconditioning (IPC) has been shown to protect the liver against ischemia-reperfusion (I/R) injuries. However, ischemic post-conditioning has received little attention. The aim of the present study was to quantify and compare the hepato-protective properties of IPC and IPO, for the first time, using unbiased design-based stereological methods.

Methods

We divided 67 rats into four groups: sham, liver ischemia (LI), IPC, and IPO. Rats were subjected to 60 min LI, followed by 4- or 24-h reperfusion. We performed quantification of (NVR) and apoptotic cell profile number.

Results

We observed no significant differences in NVR between ischemic groups after 4 h. After 24-h reperfusion, NVR had increased to 70 % in the LI group, compared with 51 % (P?=?0.02) and 49 % (P = 0.01) in the IPC and IPO groups, respectively. After 4-h reperfusion, the apoptotic cell number was significantly higher in all ischemic groups than in the sham group; we detected no difference between ischemic groups. After 24-h reperfusion, we detected a significantly lower number of apoptotic cell profiles in the IPC group than in the LI group (P = 0.02). The mean number of apoptotic cell profiles decreased insignificantly in the IPO group (P = 0.06). Liver parameters were at all time comparable between groups.

Conclusions

After I/R, IPC and IPO reduce the degree of hepatocellular injury. Both methods are equally efficient at preventing hepatocellular necrosis. Furthermore, apoptosis is significantly lower after IPC.