Paecilomide, a new acetylcholinesterase inhibitor from Paecilomyces lilacinus

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• 作者：Ana Paula C. Teles^a ; ^b ; Jacqueline A. Takahashi^b ; ^{jat@qui.ufmg.br}
• 关键词：Paecilomyces lilacinus ; OSMAC ; Acetylcholinesterase inhibition
• 刊名：Microbiological Research
• 出版年：2013
• 出版时间：6 May, 2013
• 年：2013
• 卷：168
• 期：4
• 页码：204-210
• 全文大小：325 K

文摘

Fungi are some of the most important organisms in the production of bioactive secondary metabolites. This success is related to the advances in biotechnology and also to the possibility of working with techniques such as the ¡°OSMAC¡± (one strain-many compounds) to achieve different fungal secondary metabolites profiles upon modifying the culturing conditions. Using this approach, the fungal species Paecilomyces lilacinus was cultivated in potato dextrose broth under 14 different fermentative conditions by adding the bacterium Salmonella typhimurium to the growing medium in order to provide biotic stress. S. typhimurium was added alive or after inactivation by autoclave or microwave irradiation in different stages of fungal growth. Extracts were prepared by liquid-liquid extraction using ethyl acetate, a medium polarity solvent in order to avoid extracting culturing media components. Production of fatty acids of relevance for the pharmaceutical and food industries was enhanced by the modified fermentative conditions and they were identified and quantified. The extracts were evaluated for acetylcholinesterase inhibition and the more active extract (91 ¡À 2.91 % inhibition) was prepared in large scale. From this active P. lilacinus extract, a novel pyridone alkaloid, named Paecilomide, was isolated and its structure was elucidated by modern nuclear magnetic resonance techniques and mass spectrometric analyses. Paecilomide (1) was also evaluated for acetylcholinesterase inhibition, presenting 57.5 ¡À 5.50 % of acetylcholinesterase inhibition.