Biochemical characterization of a novel antioxidant and angiotensin I-converting enzyme inhibitory peptide from Struthio camelus egg white protein hydrolysis

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• 作者：Ahmad Asoodeh^a ; ^{asoodeh@um.ac.ir" class="auth_mail" title="E-mail the corresponding author} ; Masoud Homayouni-Tabrizi^b ; Hoda Shabestarian^c ; Shamsi Emtenani^a ; Shirin Emtenani^a
• 关键词：angiotensin I-converting enzyme ; antioxidant peptide ; molecular docking ; ostrich egg white proteins
• 刊名：Journal of Food and Drug Analysis
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：24
• 期：2
• 页码：332-342
• 全文大小：1419 K

文摘

A peptide from ostrich (Struthio camelus) egg white protein hydrolysate (OEWPH) was purified, characterized, and its antioxidant and enzyme inhibitory properties were evaluated. The OEWPH was prepared using pepsin and pancreatin, and then fractionated using reversed-phase high performance liquid chromatography. The antioxidant activity of the WG-9 peptide was investigated based on its scavenging capacity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,20-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), superoxide (O₂^•−), hydroxyl (OH^•−), and lipid peroxidation inhibition. The angiotensin-converting enzyme (ACE) inhibitory activity and kinetic parameters of the peptide were determined using N-[3-(2-Furyl)acryloyl]-L-phenylalanyl-glycyl-glycine (FAPGG) as a substrate. Tandem mass spectrometry analysis of the purified peptide revealed a sequence of WESLSRLLG (MW: 1060 Da; WG-9). This peptide inhibited linoleic acid oxidation and acted as a DPPH (IC₅₀ = 15 ± 0.4 μg/mL), ABTS (IC₅₀ = 130 ± 4.5 μg/mL), superoxide (IC₅₀ = 160 ± 6.4 μg/mL), and hydroxyl (IC₅₀ = 150 ± 6.7 μg/mL) radical scavenger. The ACE-inhibitory activity and kinetic parameters of the WG-9 peptide were determined, showing an ACE inhibitory activity with IC₅₀ of 46.7 ± 1.4 μg/mL. The parameters of peptide/ACE interactions were investigated by molecule docking. Furthermore, viability assays showed that the identified peptide had no cytotoxicity against an HFLF-PI-5 cell line. In conclusion, the WG-9 peptide showed potent antioxidant and ACE-inhibitory activity.