Neuroprotective polyhydroxypregnane glycosides from Cynanchum otophyllum
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- 作者：Zhi-Min Zhao ; Zhang-Hua Sun ; Mei-Hui Chen ; Qiong Liao ; Ming Tan ; Xin-Wen Zhang ; Han-Dong Zhu ; Rong-Biao Pi ; Sheng Yin
- 关键词：Cynanchum otophyllum ; Polyhydroxypregnane glycosides ; Structure elucidation ; Neuroprotective effect
- 刊名：Steroids
- 出版年：2013
- 出版时间：October, 2013
- 年：2013
- 卷：78
- 期：10
- 页码：1015-1020
- 全文大小：722 K
文摘
Five new polyhydroxypregnane glycosides, namely cynanotosides A?E (1-5), together with two known analogues, deacetylmetaplexigenin (6) and cynotophylloside H (7), were isolated from the roots of Cynanchum otophyllum. Their structures were established by spectroscopic methods and acid hydrolysis. The neuroprotective effects of compounds 1-7 against glutamate-, hydrogen peroxide-, and homocysteic acid (HCA)-induced cell death were tested by MTT assay in a hippocampal neuronal cell line HT22. Compounds 1, 2, and 7 exhibited protective activity against HCA-induced cell death in a dose-dependent manner ranging from 1 to 30 ¦ÌM, which may explain the Traditional Chinese Medicine (TCM) use of this plant for the treatment of epilepsy.