- 作者：Andrzej Wró ; bel ; wrobelandrzej@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Tomasz Rechberger
- 关键词：Cystometry ; Depression ; Detrusor overactivity ; Methods ; Rats ; 13-cis-Retinoic acid
- 刊名：Journal of Pharmacological and Toxicological Methods
- 出版年：2016
- 出版时间：July-August 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：19-25
- 全文大小：618 K
Methods
In order to assess the 13-cis-retinoic acid impact on the behavioural parameters, after 6 weeks of intraperitoneal administration of retinoid in a dose of 1 mg/kg/day, a forced swim test and cystometry were performed, and the locomotor activity of animals was assessed. The control group received a mixture of DMSO and physiological saline at a 1:1 ratio.
Results
13-cis-retinoic acid caused cystometric parameter changes analogous to those observed in people with a urodynamic diagnosis of detrusor overactivity. The retinoid caused also an extension of the immobility time in the forced swim test which is consistent with increased depression-related behaviour, with no impact on the locomotor activity of rats. The intravenous administration of solifenacin succinate in a single dose of 0.03 mg/kg turned out to reverse changes in the cystometric parameters modified by 13-cis-retinoic acid treatment. The histopathological analysis of bladders did not show any lesions in the upper layer of the umbrella cells, urothelium or muscles. The retinoid concentration level achieved in the animals tested turned out to be identical to that occurring in humans.
Discussion
13-cis-retinoic acid can induce detrusor overactivity symptoms that are reversed by solifenacin succinate.