Overexpression of functional human oxidosqualene cyclase in Escherichia coli
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- 作者：Charlotte Kü ; rtena ; Mathias Uhlé ; na ; b ; c ; Per-Olof Syré ; na ; per-olof.syren@scilifelab.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Oxidosqualene cyclase ; E. coli ; Expression and purification ; Membrane protein ; Triterpene cyclase
- 刊名：Protein Expression and Purification
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：115
- 期：Complete
- 页码：46-53
- 全文大小：1401 K
文摘
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We report the overexpression of active human oxidosqualene cyclase in E. coli.
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A membrane extraction protocol based on a standard centrifuge was optimized.
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Our yield of 15 mg membrane protein/L E. coli culture is comparable to yeast.
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An easy to handle bacterial system will be beneficial in industrial applications.