Microvascular Tissue Oxygenation and Oxidative Metabolism Changes in the Pedicled Latissimus Dorsi Muscle During Graded Hypoxia: Correlation Between Near Infrared and ³¹P Nuclear Magnetic Resonance Spectroscopy

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• 作者：Dirk Troitzsch ; M.C.V.T. ; Rainer Moosdorf ; M.D. ; Sebastian Vogt ; M.D. ; ^{vogts@med.uni-marburg.de}
• 关键词：latissimus dorsi muscle ; microvascular tissue oxygenation ; graded hypoxia ; tissue energy metabolism ; oxygen ; 31P NMR spectroscopy ; near-infrared spectroscopy ; rabbits
• 刊名：Journal of Surgical Research
• 出版年：2012
• 出版时间：July, 2012
• 年：2012
• 卷：176
• 期：1
• 页码：337-342
• 全文大小：394 K

文摘

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Background

In this study, the microvascular tissue oxygenation and oxidative muscle metabolism during graded hypoxia and reoxygenation were examined in a rabbit model by near-infrared (NIR) spectroscopy and correlated with high-energy phosphates measured by ³¹P nuclear magnetic resonance (NMR) spectroscopy.

Materials and Methods

Graded hypoxia was performed in a New Zealand rabbit model (n = 20, 2.0?¡À 0.4 kg) by a stepwise reduction of the fraction of inspired oxygen (FiO₂) from 0.3 to 0.05 (intervention group versus control group). Recovery and reoxygenation were achieved using FiO₂ of 0.3. A noninvasive NIR spectroscopy sensor and NMR probe was positioned on the surface of the prepared pedicled latissimus dorsi muscle. Microvascular tissue oxygenation (oxyhemoglobin, HbO₂; deoxyhemoglobin, HHb) and redox state of cytochrome oxidase (CytOx) were measured by NIR spectroscopy and correlated with standard values of oxidative muscle metabolism (phosphocreatine, PCr; adenosine triphosphate, ATP) measured by time-resolved ³¹P NMR spectroscopy (4.7T).

Results

Significant correlation was found between PCr and HbO₂ (r = 0.85, P < 0.001) and HHb (r?=??.75, P < 0.001). ¦Â-ATP levels correlated significantly with CytOx (r = 0.87, P < 0.001).

Conclusions

The data suggest that changes in high-energy phosphates (PCr- and ATP-levels) correlate closely with microvascular tissue oxygenation (HbO₂, HHb, CytOx) measured by NIR spectroscopy.