Time-lapse morphokinetic assessment has low to moderate ability to predict euploidy when patient- and ovarian stimulation-related factors are taken into account with the use of clustered data analysis

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• 作者：Sezcan Mumusoglu ; M.D.^a ; Irem Yarali ; M.Sc. ; M.H.S.^d ; Gurkan Bozdag ; M.D.^a ; Pınar Ozdemir ; Ph.D.^b ; Mehtap Polat ; M.D.^d ; Lale Karakoc Sokmensuer ; M.D.^c ; Hakan Yarali ; M.D.^a ; ^d ; ^{hyarali@hacettepe.edu.tr}
• 关键词：Time-lapse ; morphokinetic parameters ; aneuploidy ; preimplantation genetic testing ; confounding ; cluster analysis
• 刊名：Fertility and Sterility
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：107
• 期：2
• 页码：413-421.e4
• 全文大小：425 K
• 卷排序：107

文摘

To study whether time-lapse morphokinetic (TLM) assessment predicts ploidy status when patient– and ovarian stimulation–related factors are taken into account.DesignRetrospective cohort study.SettingPrivate IVF clinic.Patient(s)In total, 103 consecutive patients (415 blastocysts) were included. All embryos were individually cultured in a time-lapse incubator from intracytoplasmic sperm injection up to trophectoderm biopsy. Following trophectoderm biopsy on day 5 or 6, blastocysts were vitrified and 23 TLM parameters were analyzed.Intervention(s)Correlations between patient– and ovarian stimulation–related factors and TLM parameters were tested in a multilevel mixed-effects linear regression model and assessed by means of intraclass correlation coefficient (ICC).Main Outcome Measure(s)Predictive ability of TLM parameters for euploidy.Result(s)The majority of TLM parameters had ICCs of 16%–47%. None of the patient– or ovarian stimulation–related factor had any systematic effect on any TLM parameter; however, body mass, total FSH dose, duration of infertility, number of previous cycles, antral follicle count, ovarian stimulation protocol, and E2 on the trigger day had a significant impact on some TLM parameters. With the use of multilevel mixed-effects logistic regression analysis, of the ten TLM parameters that were initially noted to be significantly different among euploid and aneuploid blastocysts in the univariate analysis, only five remained significant. However, the areas under the receiver operating characteristic curves at regression analysis were low, ranging from 0.55 to 0.63.Conclusion(s)Five TLM parameters, all related to timing of blastocyst development, have limited ability to predict euploidy when patient– and ovarian stimulation–related factors are taken into account.